Journal: Bone

Article Title: Inhibition of the mitochondrial permeability transition improves bone fracture repair

doi: 10.1016/j.bone.2020.115391

Figure Lengend Snippet: BMSCs from CypD−/− and control CypD+/+ littermates were osteoinduced for 0 or 14 days and analyzed by staining for alkaline phosphatase (ALP, A) and for mineralization with alizarin red (ARed, B) and by real-time RT-PCR (C). CypD−/− BMSCs show more intense ALP and ARed staining (A & B, respectively) and higher expression of osteogenic gene marker, Bglap (C). D) BMSCs isolated from C57BL/6J mice were osteoinduced for 14 days in the presence of NIM811 at 0.5 mM or vehicle control; and osteogenic marker gene expression was measured with real-time RT-PCR. NIM811-treated cells had significantly higher expression of Alp. BMSCs were also implanted into backs of immunocompromised nude mice for an ectopic bone formation assay (E – G). Ossicle formation was analyzed after 6 weeks; E) X-ray showing ectopic bone formation; F) Ectopic bone was measured with histomorphometry. CypD−/− BMSCs show a significant increase in ectopic bone formation; G) Histology sections of ectopic bones with boxed areas magnified showing bone (B), marrow (M), and blood vessels (V). Data are presented as fold changes over undifferentiated control CypD+/+ BMSCs. Plots show actual data points and calculated means. *, p < 0.05, determined by ANOVA followed by post-hoc analysis (A μ C) or unpaired t-test (D, F).

Article Snippet: ABH/OG-, TRAP- or immunohistochemistry (IHC)-stained slides were scanned in an Olympus VSL20 whole slide imager at 40x magnification and evaluated with VisioPharm automated histomorphometry software.

Techniques: Staining, Quantitative RT-PCR, Expressing, Marker, Isolation, Tube Formation Assay

Journal: Bone

Article Title: Inhibition of the mitochondrial permeability transition improves bone fracture repair

doi: 10.1016/j.bone.2020.115391

Figure Lengend Snippet: Histological staining using ABH/OG stain followed by histomorphometry of fractured bones was performed at various time points (A). CypD−/− mice show accelerated callus ossification and a truncated cartilaginous phase at PFD 7 and 14 (B & C). Plots show actual data points and calculated means. *, p < 0.05, determined by unpaired t-test of each time point, t-test was used because samples at each time point were collected and analyzed independently of other time points. See also supplementary Figure S6.

Article Snippet: ABH/OG-, TRAP- or immunohistochemistry (IHC)-stained slides were scanned in an Olympus VSL20 whole slide imager at 40x magnification and evaluated with VisioPharm automated histomorphometry software.

Techniques: Staining

Journal: European Heart Journal. Imaging Methods and Practice

Article Title: Assessment of HeartModel® automated left ventricular ejection fraction for patients with hypertrophic cardiomyopathy

doi: 10.1093/ehjimp/qyag003

Figure Lengend Snippet: Bland Altman plots demonstrating a test of mean difference between blinded echocardiographer read (‘STD’), Philips HeartModel® software measurements (‘Automated’) and ‘cMRI’. Upper limit of agreement (Upper LoA) and Lower limit of agreement (Lower LoA) as well as mean difference is displayed for each comparison.

Article Snippet: LV assessments were made by (i) Philips HeartModel® software model (automated), (ii) a single board-certified echocardiographer (AR) blinded to the automated assessments (standard), (iii) cardiac magnetic resonance imaging (cMRI) when available.

Techniques: Software, Comparison

Journal: European Heart Journal. Imaging Methods and Practice

Article Title: Assessment of HeartModel® automated left ventricular ejection fraction for patients with hypertrophic cardiomyopathy

doi: 10.1093/ehjimp/qyag003

Figure Lengend Snippet: Correlation plots comparing standard methods (‘STD’), Philips HeartModel® software (‘Automated’), and ‘cMRI’ read. LV EF, Left Ventricular Ejection Fraction. LV EDV, Left Ventricular End Diastolic Volume. LV ESV, Left Ventricular End Systolic Volume. Correlation co-efficient ( R 2 ) displayed for each comparison.

Article Snippet: LV assessments were made by (i) Philips HeartModel® software model (automated), (ii) a single board-certified echocardiographer (AR) blinded to the automated assessments (standard), (iii) cardiac magnetic resonance imaging (cMRI) when available.

Techniques: Software, Comparison

Journal: European Heart Journal. Imaging Methods and Practice

Article Title: Assessment of HeartModel® automated left ventricular ejection fraction for patients with hypertrophic cardiomyopathy

doi: 10.1093/ehjimp/qyag003

Figure Lengend Snippet: Analysis of automated tracing errors. Representative B-mode imaging frames are shown. Automated tracings represent unedited outputs from the HeartModel® software. Sources of error were identified by manual review of the automated tracings. EDV, end diastolic volume; ESV, end systolic volume; LVEF, left ventricular ejection fraction.

Article Snippet: LV assessments were made by (i) Philips HeartModel® software model (automated), (ii) a single board-certified echocardiographer (AR) blinded to the automated assessments (standard), (iii) cardiac magnetic resonance imaging (cMRI) when available.

Techniques: Imaging, Software

Journal: European Heart Journal. Imaging Methods and Practice

Article Title: Assessment of HeartModel® automated left ventricular ejection fraction for patients with hypertrophic cardiomyopathy

doi: 10.1093/ehjimp/qyag003

Figure Lengend Snippet: Correlation plot comparing Left Ventricular Ejection Fraction (LVEF) by standard methods (‘STD’) with Philips HeartModel® Software (‘Automated’). Correlation co-efficient ( R 2 ) displayed. Patients who had an STD LVEF above 50% but an Automated LVEF of 50% or below are represented as red dots. Patients who had a STD LVEF of 50% or below, but an automated LVEF above 50% are represented as orange dots. 50% LVEF cut off threshold denoted by brown lines.

Article Snippet: LV assessments were made by (i) Philips HeartModel® software model (automated), (ii) a single board-certified echocardiographer (AR) blinded to the automated assessments (standard), (iii) cardiac magnetic resonance imaging (cMRI) when available.

Techniques: Software